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MR Vol.13 No.4 indexに戻る
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MODERN RHEUMATOLOGY
Vol.13 No.4 |
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Characterization of anticytoplasmic antibodies
in patients with systemic autoimmune diseases |
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| Hiroaki Ida1 , Mingguo Huang1, Ayumi Hida1, Tomoki
Origuchi2, Atsushi Kawakami1, Kiyoshi Migita1, Mitsuhiro Tsujihata3,
Tsuneyo Mimori4 and Katsumi Eguchi1 |
(1) First Department of Internal Medicine, Nagasaki
University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501,
Japan
(2) Nagasaki University School of Health Sciences, Nagasaki, Japan
(3) Department of Internal Medicine, Nagasaki Kita Hospital, Nagasaki, Japan
(4) Department of Rheumatology and Clinical Immunology, Graduate School of Medicine,
Kyoto University, Kyoto, Japan |
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| Received: 15 October 2002 Accepted: 14 February
2003 |
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| Abstract |
| We characterized the cytoplasmic staining patterns
identified by indirect immunofluorescence (IF) using human epithelial
(HEp-2) cells as substrates, and identified autoantigens using enzyme-linked
immunosorbent assay (ELISA) and cognate RNA immunoprecipitation techniques
in cytoplasmic antibody-positive sera (CA(+)) in patients with systemic
autoimmune diseases. Twenty-three sera (3.7%) of 630 patients were
found to have a cytoplasmic staining pattern by IF on HEp-2 cells.
The fine-pattern IF specificities were as follows: 12 diffuse fine
speckled; 7 coarse granular filamentous speckled; 2 diffuse coarse
speckled; 1 condensed large speckled; 1 cytoskeletal. No relationship
was found between the staining patterns and the diseases. Anti-SS-A
antibodies and antimitochondrial (M2) antibodies were detected by
ELISA in 6 and 4 sera, respectively, and antismooth muscle antibody
was detected by IF in 1 serum. In RNA immunoprecipitation assays,
6, 11, 3, and 1 patients had antibodies that recognized aminoacyltransfer
RNA (tRNA) synthetases (including 2 EJ, 2 PL-7, 1 PL-12, and 1 unidentified
tRNA-related), SS-A, ribosomes, and SRP, respectively. Moreover,
several other autoantigens were detected by Western blotting using
human epithelial (HEp-2) cell lysates. This study suggests that autoantibodies
against tRNA synthetases, SS-A, ribosomes, mitochondria, and other
autoantigens are present in CA(+) sera from patients with a variety
of systemic autoimmune diseases. |
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| Key words |
| Autoantibody - Cytoplasmic antibody (CA) - Human
epithelial (HEp-2) cells - Systemic autoimmune disease - Transfer
RNA (tRNA) |
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