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MR Vol.13 No.3 indexに戻る
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MODERN RHEUMATOLOGY
Vol.13 No.3 |
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CD154 expression and mRNA stability of activated
CD4-positive T cells in patients with systemic lupus erythematosus
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| Makiyo Takaya1 , Naoto Tamura1, Kazunori Kato2,
Shigeto Kobayashi1, Kazuhiko Haruta1, 3, Michiko Tajima1, Mutsuko
Hara4, Kwang-Seok Yang1, Hiroshi Tsuda1 and Hiroshi Hashimoto1 |
(1) Department of Rheumatology, Juntendo University
School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan
(2) Department of Molecular Medicine, Sapporo Medical University, Sapporo, Japan
(3) Research Laboratory, Zenyaku Kogyo Co., Ltd., Tokyo, Japan
(4) Division of Biochemistry, Central Laboratory for Medical Science, Juntendo
University School of Medicine, Tokyo, Japan |
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| Received: 25 September 2002 Accepted: 09 December
2002 |
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| Abstract |
| The expression of CD154 (CD40 ligand) on activated
CD4+ T cells is known to be transient and tightly regulated for antigen-specific
immune responses, and is increased and prolonged among patients with
systemic lupus erythematosus (SLE). We investigated the regulation
of CD154 expression by determining the protein and mRNA expression
with PMA and ionomycin stimulation in CD4+ T cells, and confirmed
their increase and prolongation in SLE T cells. Treatment with actinomycin
D, a transcription inhibitor, after PMA and ionomycin stimulation
was performed, and the findings revealed that the stability of CD154
mRNA increased significantly in activated SLE T cells compared with
that of controls. However, alternations or abnormal sequences were
not identified in the 3 untranslated region, including AU-rich elements
and CU-rich sequences, while their partial involvement in the posttranscriptional
regulation of CD154 mRNA stability has been reported. With 96h culture
in vitro, the destabilization of CD154 mRNA was demonstrated, resulting
in a corresponding decrease and normalization of surface expression
on activated SLE T cells. We speculate that the CD154 expression
on T cells from SLE patients may be increased and prolonged, with
mRNA stabilization being related to a continuous stimulation in vivo. |
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| Key words |
| CD154 (CD40L) - mRNA stability -
Systemic lupus erythematosus (SLE) - 3 untranslated region |
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