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MR Vol.13 No.2 indexに戻る
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MODERN RHEUMATOLOGY
Vol.13 No.2 |
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CD44 expression in synovial lining and cartilage
of rat knees induced by intraarticular injection of synthetic lipid
A
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| Akihiro Honda, Tomihisa Koshino, Takeshi
Hayashi, Toshitaka Takagi, Renzo Okamoto, Tomoyuki Saito |
| Department of Orthopaedic Surgery, Yokohama City
University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama
236-0004, Japan |
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| Abstract |
| To study the effect of synthetic lipid A on
the expression of the adhesion molecule CD44 in synovium and cartilage,
synthetic lipid A (10?&mgr;g/ml, 0.5?ml) diluted in 0.025% triethylamine
(TEA) was injected into the left knee of male Wistar rats. The equivalent
volume of 0.025% TEA was injected simultaneously into the right knee
of the same rat as a control. The numbers of infiltrating neutrophils
and mononuclear cells in the synovium were counted, and the expression
of CD44 was detected immunohistochemically. Infiltration of neutrophils
in the synovium of the knee reached a maximum at 12?h after intraarticular
injection of lipid A (78.4 ± 5.6 cells/400× field), and had subsided
at 7 days after injection (2.1 ± 0.6). CD44 expression in the lining
cells of the synovium was detected 24?h after injection (2.5 ± 0.3
cell layers), and it lasted 7 days after injection (2.6 ± 0.4 cell
layers). CD44 expression in cartilage started at 24?h (69.0% ± 4.6%
positive area of specimen) and lasted 7 days after injection (27.3%
± 3.3%). Intraarticular injection of lipid A was proven to induce
acute arthritis and CD44 expression in the synovial lining layers
and articular cartilage |
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| Key words |
| Acute inflammation ・ CD44 ・ Intraarticular
injection ・ Lipid A |
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