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MR Vol.13 No.2 indexに戻る
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MODERN RHEUMATOLOGY
Vol.13 No.2 |
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The levels of leukemia inhibitory factor in synovial
tissues of patients with rheumatoid arthritis: inflammation and other
proinflammatory cytokines |
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| Hiroyuki Enomoto , Seiji Saito , Hiroshi
Yabe , Yoshiaki Toyama, Taisuke Tomatu |
| Institute of Rheumatology, Tokyo Women's Medical
University, 10-22 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-0054, Japan |
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| Abstract |
| To clarify the effect of leukemia inhibitory
factor (LIF) on the destruction of rheumatoid arthritis (RA) joints,
we investigated the production of LIF and the expression of LIF mRNA
in synovial tissues from patients with RA and osteoarthritis (OA).
Synovial fluids from RA were used to measure the LIF concentrations
using enzyme-linked immunosorbent assay (ELISA). Immunohistochemistory
and RT-PCR were used to examine the expression of LIF by synovial
cells. LIF mRNA was detected in all cases in RA synovial cells. Although
LIF protein was detected only in 20 cases (19%) in RA synovial fluids,
LIF concentration in the synovial fluids significantly correlated
with the peripheral leukocyte count (P < 0.001) and C-reactive
protein (CRP) (P < 0.01). Moreover, levels of IL-1&bgr;, IL-6,
and IL-8, but not TNF-&agr;, were significantly correlated with
LIF in the RA synovial fluids. LIF production was promoted by IL-1&bgr;
and TNF-&agr; stimulation; in contrast, IL-1 ra and IL-4 were
found to markedly decrease LIF production by cultured synovial cells.
LIF appeared to be a cytokine produced by RA synovium leading to
a proinflammatory secretion profile. Moreover, IL-4 and IL-1 ra may
represent attenuated activity for reducing the effect of the destruction
of joints by LIF. |
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