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MR Vol.13 No.1 indexに戻る
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MODERN RHEUMATOLOGY
Vol.13 No.1 |
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Expression of wnt signaling molecules in the
synovial membranes of rabbit ankle joints injected with Enterococcus
faecalis cell fractions |
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| S. Sakurai1, T. Hayashi2, S. Iwasaki3,
T. Kohno2, M. Kohno4 |
(1)Department of Molecular Genetics, Kohno Clinical
Medicine Research Institute, 3-4-4 Kitashinagawa, Shinagawa-ku, Tokyo
140-0001, Japan
(2)Tomidahama Hospital Research Division, Yokkaichi, Japan
(3)Second Department of Pathology of Aichi Medical University, Aichi, Japan
(4)Kohno Clinical Medicine Research Institute, Tokyo, Japan |
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| Abstract |
| Abstract The cell adhesion molecule #-catenin
mediates the transduction of wnt signals to various downstream events
such as gene expression, cell proliferation, and cell adhesion. In
this study, the results of reverse transcriptase polymerase chain
reaction (RT-PCR) amplification showed that wnt1 and #-catenin expression
increased in response to E. faecalis, and that the increases in wnt1
and #-catenin activated transcription of cyclin D1. Immunohistochemistry
also showed that stimulating wnt1 with E. faecalis cell fractions
leads to the stabilization and accumulation of #-catenin in the synovial
membrane. On the other hand, the results of RT-PCR showed overexpression
of various inflammatory cytokines, including IL-1-#, TGF-#, TNF-!,
and IL-6, in the synovial membrane of joints injected with E. faecalis
cell fractions. These findings suggest that expression of cyclin
D1 is strongly dependent on #-catenin/Tcf and has a direct effect
on the proliferation of synoviocytes, such as IL-1-# and TGF-#, that
leads to inhibition of Fas-antigen-mediated apoptosis of synovial
cells. |
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| Key words |
| Key words Cytokine ・ &bgr;-Catenin ・ Enterococcus
faecalis ・ Synovium ・ wnt |
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