 |
MR Vol.13 No.1 indexに戻る
 |
MODERN RHEUMATOLOGY
Vol.13 No.1 |
 |
|
|
 |
|
|
Human autoantibodies as reagents in biomedical
research |
|
|
| T. Kakinuma1, B.-H. Toh2, J. W. Sentry2 |
(1)Department of Orthopaedic Surgery, Faculty
of Medicine, Kyoto University, Kyoto, Japan
(2) Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria
3181, Australia |
| |
| Abstract |
| Abstract Autoantibodies are typically associated
with autoimmune diseases. In some instances the association of specific
autoantibodies to a specific autoimmune disease have made their detection
invaluable in clinical diagnosis. However, certain autoantibodies
show no specific disease association and therefore have limited clinical
utility. Nevertheless, autoantibodies are powerful tools for identification,
characterization, and functional studies of their cognate antoantigens.
In addition, the study of autoantibodies and their cognate autoantigens
in human disease and in experimental animal models can provide valuable
insight into disease mechanisms and the factors that ameliorate or
reverse disease. This review will focus on three specific sets of
autoantibodies, which were initially selected for investigation purely
on the basis of their novel patterns of reactivity. These were observed
when they were applied to a diagnostic HEp-2 test slide for antinuclear
antibody detection by indirect immunofluorescence. The target autoantigens
were identified as the trans-Golgi network protein GOLGA4 (Golgin
245 or p230), the early endosome antigen-1 (EEA1) and a yet to be
identified and fully characterized phosphoepitope(s) restricted to
chromosomal arms of condensed mitotic/meiotic chromosomes (MCA1).
This laboratory has exploited sera which are reactive to these autoantigens
for their identification and characterization, and in functional
studies. This review highlights the uses of autoantibodies that may
have limited diagnostic or prognostic utility, but are nonetheless
novel reagents in the prosecution of molecular cell biology. |
| |
| Key words |
| Key words Autoantibody ・ Autoimmune disease
・ Molecular biology research |
|
|
 |
|
|
