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MR Vol.12 No.1 indexに戻る
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MODERN RHEUMATOLOGY
Vol.12 No.1 |
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Anti-Ki antibodies recognize an epitope
homologous with SV40 nuclear localization signal: clinical significance
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reactivities in various immunoassays |
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| T. Yano1, Y. Takasaki1, K. Takeuchi1, K. Hirokawa1,
K. Yamanaka1, H. Hashimoto1 |
| (1)Department of Internal Medicine and Rheumatology,
Juntendo University, School of Medicine, 2-1-1 Hongo, Bunkyo-ku,
Tokyo 113-8421, Japan |
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| Abstract |
| Abstract We studied the characteristics of
anti-Ki antibodies that react with a synthetic peptide (KILT) which
has homology with SV40 large T antigen nuclear localization signal
(SV40 T NLS). Immunoreactivity of antibodies to KILT was tested
by enzyme-linked immunosorbent assay (ELISA), and this reactivity
was compared with results obtained by immunoblotting using purified
Ki and ELISA using purified or recombinant Ki antigen. The clinical
significance of anti-KILT antibodies in lupus patients was also
studied. Twenty percent of anti-Ki sera reacted with KILT, and
all sera that reacted with KILT recognized both purified and recombinant
Ki antigen in all our assay systems. A significant correlation
was observed between reactivities in ELISA using KILT and with
these using purified and recombinant Ki. Some sera with low titers
by double immunodiffusion (DID) reacted with KILT, whereas high-titer
anti-Ki sera showed a tendency to react with Ki antigen in different
assays. The prevalence of discoid rash and sicca complex was higher
in the anti-KILT-positive group. The amino acid sequence homologous
to SV40 T NLS is an immunologically active epitope on the Ki antigen;
reactivity to this epitope is associated with characteristic clinical
features allied with anti-Ki antibodies in lupus patients. |
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| Key words |
| Key words Anti-Ki antibody ・ Ki antigen ・
SV40 large T antigen ・ Systemic lupus erythematosus (SLE) |
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