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MR Vol.11 No.4 indexに戻る
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MODERN RHEUMATOLOGY
Vol.11 No.4 |
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Osteoclast differentiation factor induces synovial
macrophage osteoclast differentiation in rheumatoid arthritis |
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| O. Kudo1, Y. Fujikawa1, T. Hirayama1, T.
Torisu1 |
| (1) Department of Orthopedic Surgery, Oita
Medical University, 1-1 Idaigaoka, Hasama-machi, Oita-gun, Oita
879-5593, Japan |
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| Abstract |
| Abstract The aim of this study was to clarify
the role of osteoclast differentiation factor (ODF) and osteoprotegerin
(OPG) in synovial macrophage?osteoclast differentiation. Synovial
macrophages were cultured in the presence of macrophage-colony-stimulating
factor (M-CSF) and/or ODF. OPG was added to cocultures of synovial
macrophages and UMR106. The cultures on glass coverslips were stained
with osteoclast-associated markers, tartrate-resistant acid phosphatase
(TRAP), and vitronectin receptor (VNR), as well as macrophage-associated
markers CD11b and CD14. Functional evidence of osteoclast formation
was determined by a resorption pit assay. To investigate whether
rheumatoid arthritis (RA) synovial cells expressed messenger RNA
(mRNA) for ODF, OPG, and the receptor activator of NF-3B (RANK),
we performed a polymerase chain reaction (PCR) analysis. The addition
of M-CSF or ODF alone induced TRAP-positive multinucleated cell formation.
Resorption pits were rarely detected with M-CSF alone. ODF was capable
of inducing bone resorption and enhancing osteoclastogenesis, as
well as bone resorption in the presence of M-CSF. In the coculture
system, both osteoclast formation and bone resorption were inhibited
by OPG in a dose-dependent manner. In all experiments, synovial cells,
including macrophages and fibroblasts, expressed the mRNA for RANK,
ODF, and OPG. Our findings suggest that ODF plays a role in regulating
RA synovial macrophage?osteoclast differentiation, and that synovial
cells might have the ability to produce ODF. OPG might be further
developed as a new strategy for treating bone destruction in RA joints. |
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| Key words |
| Key words Bone destruction ・ Osteoclast ・
Osteoclast differentiation factor (ODF) ・ Osteoprotegerin (OPG)
・ Rheumatoid arthritis (RA) |
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