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MR Vol.11 No3 indexに戻る
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MODERN RHEUMATOLOGY
Vol.11 No.3 |
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Analysis of autoantibodies to cell cycle-associated
antigens
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| H. Fujinaga , K. Takeuchi1, K. Kaneda1, Y.
Takasaki1, H. Hashimoto1 |
(1) Department of Internal Medicine and Rheumatology,
Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku,
Tokyo 113-8421, Japan
(2) Department of Japanese Oriental Medicine, Toyama Medical and Pharmaceutical
University, Toyama, Japan |
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| Abstract |
| Abstract To determine the specificities of
autoantibodies targeting cell cycle-associated antigens in rheumatic
diseases, we studied 30 sera which were obtained from patients
visiting our hospital and which exhibited a variegated speckled
pattern in indirect immunofluorescence (IF). The immunoreactivities
of the sera were analyzed by Western blotting (WB) and IF. Various
reactivities to cellular components were observed in IF. The sera
reacted with proteins of various molecular weights in WB. Serum
OH from a patient with rheumatoid arthritis showed fine speckled
nucleoplasmic and nucleolar staining at interphase, discrete dot
staining associated with chromosomes and the midbody at mitosis,
and reacted with a 34-kD polypeptide in WB. The target antigen
was different from proliferating cell nuclear antigen (PCNA). The
34-kD antigen was characterized by IF using double staining procedures
and cell synchronization. The results showed that the expression
of the antigen was mainly observed between the late G1 and M-phases.
This study indicated that various cell-cycle-associated antigens
were recognized in sera from patients with rheumatic diseases,
and suggested that a 34-kD antigen recognized by serum OH was a
novel cell cycle-associated autoantigen. |
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| Key words |
| Key words Autoantibody ・ Cell cycle ・ CENP-F
・ Na antigen ・ Proliferating cell nuclear antigen (PCNA) |
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