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MR Vol.11 No.1 indexに戻る
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MODERN RHEUMATOLOGY
Vol.11 No.1 |
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Effects of mechanical vibration on DNA and proteoglycan
syntheses in cultured articular chondrocytes
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| J. Liu1, I. Sekiya1, K. Asai2, T. Tada3, T. Kato2,
N. Matsui1 |
(1)Department of Orthopaedic Surgery, Nagoya
City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku,
Nagoya 467-8601, Japan
(2)Department of Bioregulation Research, Nagoya City University Medical School,
Nagoya, Japan
(3)Department of Pathology, Nagoya City University School of Nursing, Nagoya,
Japan |
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| Abstract |
| Abstract The objective of this study was to
determine the effects of mechanical vibration loading on DNA and
proteoglycan syntheses in cultured rabbit articular chondrocytes.
Chondrocyte culture plates were placed in a vibratory apparatus
and subjected to a mechanical vibratory load at various frequencies
and periods during culture. Mechanical vibration was applied at
a sinusoidal waveform of 1.4 G-acceleration with frequencies of
200, 300, 400, 800, and 1600 Hz. 3H-thymidine and H235SO4 incorporation
were used to detect radiolabeled DNA and proteoglycan syntheses,
respectively. A frequency of 300 Hz showed a time-dependent augmentation
of DNA synthesis and gave a maximal increase on day 3 with periodic
vibration (8 h per day), and at 72 h or longer with continuous
vibration. It also promoted proteoglycan synthesis in long-term
cul-ture (from 3 to 15 days) by periodic vibration. However, frequencies
above 400 Hz suppressed biosynthesis. These results suggest that
mechanical vibration at certain frequencies may modulate the biosynthetic
response of articular chondrocytes. |
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| Key words |
| Key words Articular chondrocyte ・ DNA synthesis
・ Frequency ・ Mechanical vibration ・ Proteoglycan synthesis |
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