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MR Vol.10 No.3 indexに戻る
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MODERN RHEUMATOLOGY
Vol.10 No.3 |
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Atypical morphological characteristics and surface
antigen expression of Burkholderia pseudomallei in naturally infected
human synovial tissues |
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| R. Nanagara1, K. Vipulakorn2, S. Suwannaroj1,
H.R. Schumacher Jr3 |
(1)Department of Medicine, Faculty of Medicine,
KhonKaen University, KhonKaen 40002, Thailand
(2)Department of Orthopedics, KhonKaen University, KhonKaen, Thailand
(3)Veteran Affairs Medical Center, Philadelphia, and University of Pennsylvania,
Philadelphia, USA |
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| Abstract |
| Abstract Burkholderia pseudomallei is the causative
agent of melioidosis, a disease that frequently runs a protracted
course and is very difficult to eradicate. The mechanisms that this
organism uses to escape from host defense mechanisms and antibiotics
are not well understood. The aim of the study was to compare the
morphological characteristics and surface antigen expression of B.
pseudomallei in naturally infected human synovial tissues with the
characteristics of bacteria grown in culture media. Immunoelectron
microscopic study was performed in four synovial biopsies taken from
four B. pseudomallei septic arthritis patients. Colonies of pathogenic
B. pseudomallei collected from culture media were used as positive
controls. Polyclonal antibody to whole cell B. pseudomallei was used
as a primary antibody. Complete bacteria-like particles were demonstrated
both extracellularly and intracellularly in all four synovial specimens.
The intracytoplasmic location of B. pseudomallei and mononuclear
phagosome containing microcolony-like structures were demonstrated.
B. pseudomallei found in the synovial membrane samples were mostly
atypical, with fewer cytoplasmic electron lucent granules. Immunogold
staining of bacterial surface antigens was weaker than staining of
positive controls. We demonstrated atypical forms of B. pseudomallei
and evidence for suppression of its surface antigens in naturally
infected human synovial tissues. This adaptation may help bacteria
to survive despite host immune surveillance and treatment with antibiotics. |
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| Key words |
| Key words Septic arthritis ・ Surface antigens
・ Ultrastructure ・ Burkholderia pseudomallei ・ IEM |
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