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MR Vol.10 No.1 indexに戻る
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MODERN RHEUMATOLOGY
Vol.10 No.1 |
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Effects of growth factors and cytokines
on proteoglycan and collagen synthesis by chondrocytes in guinea
pigs with spontaneous
osteoarthritis |
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| T. Miyashita, J. Ryu, S. Saito, A. Sakamoto |
| (1) Department of Orthopedic Surgery, School
of Medicine, Nihon University, 30-1 Oyaguchikami-machi, Itabashi-ku,
Tokyo 173-8610, Japan |
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| Abstract |
| Abstract We examined the effects of various
growth factors and cytokines on proteoglycan (PG) and collagen
synthesis by chondrocytes isolated from osteoarthritic and normal
articular cartilage of Hartley strain guinea pigs. The guinea pig
represents a useful animal model of spontaneous osteoarthritis
(OA). Cartilage tissue samples were obtained from the knee joints
of under-3-month-old guinea pigs (control group) as well as 5-
to 8-month-old guinea pigs with OA changes (OA group). Chondrocytes
were isolated enzymatically and maintained in suspension culture.
Growth factor addition groups were then prepared from both the
OA group and the control group, using the factors TGF-#, bFGF,
and IGF-1 (1.25 ng/ml each). Cytokine addition groups were also
prepared using IL-1! and IL-1# (10 ng/ml each). An addition group
was also prepared for sodium hyaluronate (HA) (500 7g/ml). In each
group, 35S was added as a PG metabolic marker, 3H-proline was added
as a collagen metabolic marker, and the groups were cultured. Next,
35S and 3H-proline uptake was measured by a liquid scintillation
counter. The results revealed that (1) both PG synthesis and collagen
synthesis were promoted significantly more in OA chondrocytes than
in normal chondrocytes; (2) with the addition of growth factors,
PG and collagen synthesis was enhanced in OA chondrocytes; and
(3) PG synthesis and collagen synthesis were inhibited in both
normal and OA chondrocytes with the addition of IL-1! and -#. This
result suggests that the repair function is activated more in OA
chondrocytes than in normal chondrocytes, thereby promoting the
synthesis of the cartilage matrix by chondrocytes. This synthesizing
capability is enhanced and acts to effectively repair degenerative
articular cartilage further through the addition of growth factors. |
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| Key words |
| Key words Chondrocyte ・ Growth factor ・ Cytokine ・ Guinea pigs
・ Osteoarthritis |
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