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MR Vol.10 No.1 indexに戻る
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MODERN RHEUMATOLOGY
Vol.10 No.1 |
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Enhanced cytokine production from human
macrophages stimulated by polyethylene particles retrieved from
interface membranes
after failed total hip arthroplasty |
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| J. Chiba, K. Inoue |
| (1)Department of Orthopaedic Surgery, Daini
Hospital, Tokyo Women's Medical University, 2-1-10 Nishiogu, Arakawa-ku,
Tokyo 116-8567, Japan |
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| Abstract |
| Abstract Little is known about the specific effects
of the ultra-high molecular weight polyethylene (UHMWP) debris that
is obtained from human interface membranes after revision for a failed
total hip arthroplasty. This paper reports the effects of retrieved
polyethylene particles on human monocyte/macrophages (M/M). Macrophages
were prepared from normal human peripheral blood by the conventional
Ficoll-Hypaque method. Polyethylene wear debris was obtained from
human interface membranes and prepared by the method of papain digestion.
Human M/M were dispensed at 1.0 2 106 cells/well in a 24-well culture
plate. UHMWP and latex particles were added immediately after plating
the cells and directly onto the cells (1 2 106 cells/well) at final
particle concentrations of 100 7g/well, 200 7g/well, and 500 7g/well.
At the end of 24 h incubation, the culture supernatant was removed
and assayed for IL-1#, IL-6, and TNF-! activities by ELISA. Cellular
morphology and architecture were studied using light and electron
microscopy. Human M/M cultured with retrieved UHMWP particles caused
significantly more IL-1#, IL-6, and TNF-! release than macrophages
cultured with latex (P < 0.05). The addition of latex and polyethylene
particles to human M/M resulted in a dose-dependent increase in IL-1#,
IL-6, and TNF-! release. Electron microscopy revealed that 90% of
the UHMWP particles were less than 1 7m diameter. The average particle
size was approximately 0.7 7m diameter (range 0.1-15 7m). Human M/M
exposed to PE particles demonstrated extensive filopodia formation
as compared with the cells exposed to latex particles. In summary,
we have demonstrated that polyethylene particles isolated from interfacial
membranes obtained at revision surgery are potent stimulators of
human M/M. |
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| Key words |
| Key words Human monocyte/macrophages ・ Ultra-high
molecular weight polyethylene (UHMWP) ・ Interface membrane tissue
・ Total hip arthroplasty
(THA) |
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