Vol.22 No.3

Original Article

Acute phase response and oxidative stress status in familial Mediterranean fever (FMF)

Authors

Savas Guzel1 , Gulnur Andican2 , Arzu Seven2 , Mahmure Aslan2 , Murat Bolayirli3 , Eda Celik Guzel4 , Vedat Hamuryudan5

  • Department of Biochemistry, Medical Faculty, Namik Kemal University, Tekirdag, Turkey
  • Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
  • Fikret Biyal Central Research Laboratory, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
  • Department of Family Medicine, Medical Faculty, Namik Kemal University, Tekirdag, Turkey
  • Department of Internal Medicine, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
Received:

25 May 2011

Accepted:

16 August 2011

Published online:

23 September 2011

Full Text

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Abstract

We aimed to determine acute phase response (APR) and oxidative stress in patients with familial Mediterranean fever (FMF) and compare these characteristics with those in healthy controls; 20 patients with FMF and 15 healthy controls were enrolled in the study. The erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP), fibrinogen, and leukocyte levels were determined as markers of APR. Thiobarbituric acid reactive substances (TBARS), conjugated diene, and lipid hydroperoxide levels were measured as markers of lipid peroxidation. Carbonyl group and thiol (T-SH) levels were analyzed to determine the oxidative damage to proteins, and 8-hydroxy-2-deoxyguanosine (8-OHdG) was measured to reflect DNA oxidation. The erythrocyte glutathione (GSH) level, and glutathione peroxidase (GSH-Px), CuZn superoxide dismutase (CuZn SOD), and catalase activities were measured as markers of antioxidant status. Conjugated diene (p<0.001) and carbonyl group (p<0.05) levels were significantly higher and GSH-Px activity (p<0.01) was significantly lower in FMF patients compared with controls. FMF patients in the attack period (n = 8) had significantly higher CRP, ESR, fibrinogen, and leukocyte levels (p<0.001) than patients in the attack-free period (n = 12). The T-SH level (p<0.05) was significantly higher and CuZn SOD activity was significantly lower (p<0.05) in FMF patients in the attack period. The findings revealed upregulated APR during the attack period in FMF patients and enhanced oxidative stress in the FMF patients as compared to controls.

Key words

Familial Mediterranean fever - Acute phase response - Oxidative stress - Antioxidant status