Vol.20 No.2

Original Article

Administration of human immunoglobulin suppresses development of murine systemic vasculitis induced with Candida albicans water-soluble fraction: an animal model of Kawasaki disease

Authors

Kei Takahashi1 , Toshiaki Oharaseki1 , Yuki Yokouchi1 , Noriko N. Miura2 , Naohito Ohno2 , Akiko I. Okawara3 , Hisao Murata1 , Shiro Naoe1 , Kazuo Suzuki4,5

  • Department of Pathology, Toho University Ohashi Medical Center, 2-17-6 Ohashi, Meguro, Tokyo 153-8515, Japan
  • Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji Tokyo, 192-0392, Japan
  • Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku Tokyo, 162-8640, Japan
  • Department of Immunology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan
  • Inflammation Program, Department of Immunology, Chiba University Graduate School of Medicine, Chiba, Chiba 260-8670, Japan
Received:

12 May 2009

Accepted:

23 October 2009

Published online:

27 November 2009

Full Text

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Abstract

We investigated the inhibitory effect of human immunoglobulin (h-Ig) on the development of coronary arteritis in a murine model of vasculitis induced with a Candida albicans water-soluble fraction (CAWS). CAWS was intraperitoneally injected to C57BL/6 mice for 5 days. Then h-Ig was administered according to various schedules. The animals were sacrificed in week 5, and the status of vasculitis in the coronary arteries and the aortic root was investigated histologically. The groups in which h-Ig was administered for 5 days from day 3 and from day 5 of the experiment showed a significant reduction in the incidence of panvasculitis. In addition, the scope and severity of the inflammation of the aortic root and the coronary arteries were reduced in both groups. In the group administered h-Ig for 5 days from day 1 and the group administered a high dose of h-Ig once on day 1 or day 3, no suppression of development of vasculitis was observed. The h-Ig acted by suppressing the generation and progression of vasculitis in this CAWS-induced murine vasculitis model.

Key words

Candida albicans - Immunoglobulin - Kawasaki disease - Mouse - Systemic vasculitis