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MODERN RHEUMATOLOGY Vol.13 No.4
>MR13-4
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| Characterization of anticytoplasmic antibodies in patients with systemic autoimmune diseases |
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| Hiroaki Ida1 , Mingguo Huang1, Ayumi Hida1, Tomoki Origuchi2, Atsushi Kawakami1, Kiyoshi Migita1, Mitsuhiro Tsujihata3, Tsuneyo Mimori4 and Katsumi Eguchi1 |
(1) First Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan
(2) Nagasaki University School of Health Sciences, Nagasaki, Japan
(3) Department of Internal Medicine, Nagasaki Kita Hospital, Nagasaki, Japan
(4) Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University, Kyoto, Japan |
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| Received: 15 October 2002 Accepted: 14 February 2003 |
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| Abstract |
| We characterized the cytoplasmic staining patterns identified by indirect immunofluorescence (IF) using human epithelial (HEp-2) cells as substrates, and identified autoantigens using enzyme-linked immunosorbent assay (ELISA) and cognate RNA immunoprecipitation techniques in cytoplasmic antibody-positive sera (CA(+)) in patients with systemic autoimmune diseases. Twenty-three sera (3.7%) of 630 patients were found to have a cytoplasmic staining pattern by IF on HEp-2 cells. The fine-pattern IF specificities were as follows: 12 diffuse fine speckled; 7 coarse granular filamentous speckled; 2 diffuse coarse speckled; 1 condensed large speckled; 1 cytoskeletal. No relationship was found between the staining patterns and the diseases. Anti-SS-A antibodies and antimitochondrial (M2) antibodies were detected by ELISA in 6 and 4 sera, respectively, and antismooth muscle antibody was detected by IF in 1 serum. In RNA immunoprecipitation assays, 6, 11, 3, and 1 patients had antibodies that recognized aminoacyltransfer RNA (tRNA) synthetases (including 2 EJ, 2 PL-7, 1 PL-12, and 1 unidentified tRNA-related), SS-A, ribosomes, and SRP, respectively. Moreover, several other autoantigens were detected by Western blotting using human epithelial (HEp-2) cell lysates. This study suggests that autoantibodies against tRNA synthetases, SS-A, ribosomes, mitochondria, and other autoantigens are present in CA(+) sera from patients with a variety of systemic autoimmune diseases. |
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| Key words |
| Autoantibody - Cytoplasmic antibody (CA) - Human epithelial (HEp-2) cells - Systemic autoimmune disease - Transfer RNA (tRNA) |
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